Phenyldiimide, Hemoglobin, and Heinz Bodies

Phenyldiimide, hemoglobin, and Heinz bodies.

Phenylhydrazine is oxidized stoichiometrically by two equivalents of ferricyanide to produce phenyldiimide and ferrocyanide. This reaction permits accurate determination of the absorption maxima and molar absorption coefficients of phenyldiimide in aqueous solution. These values are identical or extremely similar to those of phenyldiimide produced by decarboxylation of phenylazoformic acid. Kno...

Fluorescent cytoplasm and Heinz bodies of hemoglobin Köln erythrocytes: evidence for intracellular heme catabolism.

Hb Köln, one of the common mutant hemoglobins responsible for unstable hemoglobin disease, was found to be degraded to a fluorescent yellow pigment (FYP) in circulating erythrocytes. FYP is responsible for a strong green fluorescence observed in the cytoplasm and is particularly abundant in the Heinz bodies of Köln RBC. Front face fluorometry and fluorescence microscopy showed that Heinz bodies...

Fluorescent Cytoplasm and Heinz Bodies of Hemoglobin K#{246}ln Erythrocytes: Evidence for Intracellular Heme Catabolism

H EMOGLOBIN KOLN (Hb K#{246}ln) is characterized by a point mutation in its chains, in which the f198 valine is replaced by the bulkier methionine residue.1 This substitution destabilizes the hemoglobin’s T state and increases its oxygen affinity, while at the same time loosening the heme pocket and making Hb K#{246}ln subject to heme loss and to the precipitation of granular deposits, known as...

Heinz bodies interfere with automated reticulocyte counts.

Correspondence: Ignacio Español, M.D., Laboratoris Folguera, c/ Aribau 212-216, entl 3a, 08006 Barcelona, Spain. Phone: international +34-93-4143481 – Fax: international +34-932021341. was low: 20 mg/dL. A very high reticulocyte count was detected by two different automated counters (Figure 1), but only one reported a staining error. A crisis of hemolytic anemia or an error in the automated ret...

Observations on Heinz bodies in normal and splenectomized rabbits.